Effect of Wenyang Jieyu Decoction on Hippocampal Neuroplasticity in Depressed Rats Induced by Corticosterone / 中国实验方剂学杂志

ObjectiveTo observe the effect of Wenyang Jieyu decoction （WYJY） on the hippocampal structure of depressed rats with kidney-yang deficiency. MethodThe 105 SD rats were randomly divided into normal group， model group， fluoxetine group （4.17 mg·kg-1）， Xiaoyaosan group （1.88 g·kg-1）， and low-， medium- and high-dose （1.25，2.50，5.00 g·kg-1） WYJY groups，15 in each group. The depression model was induced by subcutaneous injection of corticosterone in rats except for those in the normal group and the rats were orally administered once a day for 28 days. The depression-like behaviors of rats were observed by sucrose preference test， novelty-suppressed feeding test， forced swimming test， and open field test. The morphology of hippocampal neurons was observed by hematoxylin-eosin（HE） staining， and the density of hippocampal neurons was detected by Nissl staining. The ultrastructure of hippocampal synapses was observed by transmission electron microscopy （TEM）. The expression of synaptophysin （SYP）， postsynaptic density-95 （PSD95）， and apoptosis-related protein Caspase-3 in hippocampal neurons was observed by immunohistochemistry， and bromodeoxyuridine （BrdU） and doublecortin （DCX） were used to observe the apoptosis and regeneration of hippocampal neurons. ResultWYJY could improve weight loss in depressed rats. As revealed by the behavioral tests， the model group showed depression-like behaviors， which were relieved in the WYJY groups and the positive drug groups. HE staining showed that the nuclei of hippocampal neurons in the model group were constricted， deeply stained， and sparsely arranged， while the neurons in the WYJY groups and the positive drug groups were significantly improved. Nissl staining demonstrated that the cell density of the model group was lower than that of the normal group （P<0.05）. Compared with model group， the groups with drug intervention showed increased cell density （P<0.05） and compact arrangement. According to the results in TEM， compared with normal group， the model group showed shortened synaptic active zone （P<0.05）， widened synaptic cleft （P<0.05）， and thinned tight zone （P<0.05）. Compared with model group， the groups with drug intervention showed shortened synaptic active zone （P<0.05）， narrowed synaptic cleft （P<0.05）， and thickened tight zone （P<0.05）. As displayed by the results of immunocytochemistry and immunofluorescence， compared with the normal group， the model group showed decreased protein expression of SYP， PSD95， BrdU， and DCX in the hippocampus （P<0.05） and increased protein expression of Caspase-3 （P<0.05）. Compared with the model group， the groups with drug intervention showed increased protein expression of SYP， PSD95， BrdU， and DCX in the hippocampus （P<0.05） and decreased protein expression of Caspase-3 （P<0.05）. ConclusionWYJY can promote the regeneration of hippocampal neurons in rats and improve the depression of rats.

Re-examination of malaria diagnosis in Hubei Provincial Malaria Diagnostic Reference Laboratory from 2017 to 2019 / 中国血吸虫病防治杂志

ObjectiveTo analyze the re-examination results of malaria cases captured from the National Notifiable Communicable Disease Reporting System in Hubei Provincial Malaria Diagnostic Reference Laboratory from 2017 to 2019, so as to pro- vide the scientific evidence for improving the malaria control capability in the province. MethodsMicroscopy and nested PCR assay were performed to re-examine the diagnosis of malaria cases registered in the National Notifiable Communicable Disease Reporting System in Hubei Provincial Malaria Diagnostic Reference Laboratory from 2017 to 2019, and the coincidences of ma- laria diagnosis and malaria parasite species were evaluated. Results A total of 410 malaria cases were reported in Hubei Province from 2017 to 2019 according to the data retrieved from the National Notifiable Communicable Disease Reporting System. Among the 407 samples re-examined by Hubei Provincial Malaria Diagnostic Reference Laboratory from 2017 to 2019, the diag- nosis 374 malaria cases were confirmed, with an overall coincidence of 91.89% (374/407) for malaria diagnosis and 89.04% (333/374) for parasite species identification. The coincidence rates of malaria diagnosis and parasite species identification were 50.00% to 100.00% and 66.67% to 100.00% in 16 cities (prefectures) of Hubei Province during the re-examinations, which both varied in regions (χ2 = 40.46 and 42.30, both P values < 0.01). The coincidence rates of Plasmodium falciparum, P. vivax, P. malariae and P. ovale identification were 95.80%, 100.00%, 58.33% and 51.92% during the re-examinations, respectively (χ2 = 76.66, P < 0.01). The consistency rate between microscopic and nested PCR results was 89.83% (362/403). Conclusions The overall diagnostic quality of malaria is high in medical institutions at all levels in Hubei Province; however, the diagnostic capability of malaria remains to be improved in some regions.

Analysis on Forensic Expertise of 49 Medical Disputes in Prenatal Examination / 法医学杂志

OBJECTIVES@#To analyze the cases of medical disputes involving prenatal examination from a point of view of forensic expertise, and to discuss the risk of medical disputes and the preventive measures.@*METHODS@#A retrospective analysis was conducted on 49 forensic expertise cases of medical disputes in prenatal examination which were identified in Academy of Forensic Science and Shanghai Di'an Forensic Science Limited Company from 2010 to 2017.@*RESULTS@#In recent years, the number of medical disputes involving prenatal examination showed an increasing trend year by year. The common causes of medical disputes were: uninformed or insufficiently informed disclosure （20 cases）; the propaganda and application of three-dimensional, four-dimensional ultrasound were not standardized （14 cases）; ultrasound examination and serological screening process were not standardized （12 cases）; no antenatal counseling （2 cases）, etc.@*CONCLUSIONS@#In order to minimize the occurrence of such medical disputes, hospitals or related associations should avoid the risk of prenatal examination through the standardization of management and operation.

Retrospective Analysis of 24 Cases of Forensic Medical Identification on Traumatic Tympanic Membrane Perforations / 法医学杂志

OBJECTIVES@#To study the case characteristics of forensic medical identification of traumatic tympanic membrane perforations, and to discuss the key points of forensic medical identification and evaluations methods for tympanic membrane perforations.@*METHODS@#Twenty-four cases of traumatic tympanic membrane perforations accepted by the Academy of Forensic Science during 2017 were retrospectively analysed. The data of perforation size, form, predilection site, healing time and healing mode were evaluated.@*RESULTS@#For the traumatic tympanic membrane perforations, the study showed that the small size of perforation （<1/2 quadrant） with irregular shape was common. The location of perforations was almost on the anterior and inferior quadrant, and centripetal migration healing was common. The healing rate within 6 weeks was up to 90%.@*CONCLUSIONS@#In the identification cases of traumatic tympanic membrane perforations, the key is to determine whether it is traumatic and whether it will heal spontaneously within 6 weeks. It is suggested to check the tympanic membrane weekly by an otic endoscope combined with acoustic impedance measurement at the sixth week, which can improve the accuracy, objectivity and scientificity of the identification.

Analysis on Injury Manners of 31 Cases of Bennett Fracture and Rolando Fracture / 法医学杂志

OBJECTIVES@#To study the injury manners of Bennett fracture and Rolando fracture and explore its identification principle of the first metacarpal base fractures.@*METHODS@#Totally 31 cases of Bennett fracture and Rolando fracture were collected. The injury manners of cases of 19 Bennett fractures and 12 Rolando fracture were classified, and various injury manners were analysed statistically.@*RESULTS@#The injury manners of the cases of 19 Bennett fracture and 12 Rolando fracture were divided into three types, including the first metacarpus hit hard objects during a punching, the first metacarpus hit hard objects when making fists and fell, or the first metacarpus was punched by hard objects when making fists.@*CONCLUSIONS@#The injury mechanism of Bennett fracture and Rolando fracture is formed by a force on the first metacarpus when making fists, which transmits to the basilar part along the vertical axis of metacarpus. The inference of injury manners should be focused on the confirmation of entrusted matters.

Epidemiologic features and space-time clustering analysis of human schistosomiasis in Hubei Province from 2015 to 2016 / 中国血吸虫病防治杂志

To investigate the space-time patterns of schistosomiasis after the stage of transmission controlled in Hubei Province, so as to provide the reference for precise controlling.The data of human schistosomiasis cases in Hubei Province from 2015 to 2016 and basic information of human schistosomiasis cases and serum antibody titer of human schistosomiasis cases in 2016 were collected and analyzed. The spatial clustering of human schistosomiasis was detected by the Flexible spatial scan statistics and Kulldorff circular scan statistic, respectively.Totally 64 819 serological positive cases from 51 counties and 1 504 stool hatching positive cases from 17 endemic counties were reported in Hubei Province in 2015, and 39 365 serological positive cases were reported from 48 counties in 2016. All of them were identified as the research objects. No stool hatching positive cases were reported from the routine work in the whole province in 2016. There were 1 603 cases of the highest antibody titer (1∶80 or more), with the proportion of 4.07%. According to the results of Kulldorff spatial clustering analysis, there were eight and six spatial clustering areas in the distribution of serological positive cases and stool hatching positive cases in 2015, respectively. The numbers of spatial clustering areas in the distribution of serological positive cases and the cases of the highest antibody titer (1∶80 or more) were eight and five in 2016, respectively. According to the results of Flexible spatial clustering analysis, there were three and two spatial clustering areas in the distribution of serological positive cases and stool hatching positive cases in 2015, respectively. The numbers of spatial clustering areas in the distribution of serological positive cases and the cases of highest antibody titer (1∶80 or more) were two and one in 2016, respectively.The distribution of human schistosomiasis cases is not balanced, and there is spatial clustering in Hubei Province. So the key area for prevention and control is on the middle and lower reaches of the Yangtze River in the future.

Study on effect of clinical pathway implementation in advanced schistosomi-asis with ascites / 中国血吸虫病防治杂志

Objective To evaluate the effect of clinical pathway(CP)on the implementation of advanced schistosomiasis pa-tients with ascites.Methods Totally 1 129 cases of advanced schistosomiasis patients with ascites but without other complica-tions were selected randomly from the Performance Evaluation and Management System for Medical Treatment of Advanced Schistosomiasis Patients in Hubei Province from year 2011 to 2013.Among the patients,754 cases were treated by CP(CP group),and 375 cases were treated with traditional methods(NCP group),and the hospitalization days,hospitalization expens-es,medicine proportions,treatment outcomes and degrees of satisfaction and health knowledge rates of the two groups were com-pared.Meanwhile,the variation of CP was calculated.Results The average length of hospital days of the CP group and NCP group were(13.85 ± 5.60)d and(17.92 ± 5.80)d,respectively,and the average hospitalization costs of the two groups were(4 699.14 ± 1 520.59)Yuan and(5 692.01 ± 1 616.66)Yuan,respectively,both the differences were statistically significant(both P＜0.05).Also the hospitalization cost structures of the two groups were remarkably different,the composition ratios of the inspection fee and accommodation fee in the CP group were lower than those in the NCP group,but the constituent ratios of the examination fee,diagnosis and treatment fee,drug charges and other expenses were higher than those in the NCP group(all P<0.05).The awareness rate of health knowledge in the CP group was higher than that in the NCP group(P<0.05),but there were no statistically significant differences in the treatment outcome and the degree of satisfaction between the two groups(both P>0.05).The variation rate of CP was 9.02％(68/754).Conclusions The implementation of CP can decrease the days of hos-pital stay and medical expenses,improve the awareness rate on health knowledge of the patients.The CP treatment with low vari-ation rate is applicable to advanced schistosomiasis patients with ascites but without complications.

Increased Chondrocyte Apoptosis in Kashin-Beck Disease and Rats Induced by T-2 Toxin and Selenium Deficiency / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate chondrocyte apoptosis and the expression of biochemical markers associated with apoptosis in Kashin-Beck disease (KBD) and in an established T-2 toxin- and selenium (Se) deficiency-induced rat model.</p><p><b>METHODS</b>Cartilages were collected from the hand phalanges of five patients with KBD and five healthy children. Sprague-Dawley rats were administered a selenium-deficient diet for 4 weeks prior to T-2 toxin exposure. The apoptotic chondrocytes were observed by terminal deoxynucleotidyl transferase dUTP nick end labeling staining. Caspase-3, p53, Bcl-2, and Bax proteins in the cartilages were visualized by immunohistochemistry, their protein levels were determined by Western blotting, and mRNA levels were determined by real-time reverse transcription polymerase chain reaction.</p><p><b>RESULTS</b>Increased chondrocyte apoptosis was observed in the cartilages of children with KBD. Increased apoptotic and caspase-3-stained cells were observed in the cartilages of rats fed with normal and Se-deficient diets plus T-2 toxin exposure compared to those in rats fed with normal and Se-deficient diets. Caspase-3, p53, and Bax proteins and mRNA levels were higher, whereas Bcl-2 levels were lower in rats fed with normal or Se-deficiency diets supplemented with T-2 toxin than the corresponding levels in rats fed with normal diet.</p><p><b>CONCLUSION</b>T-2 toxin under a selenium-deficient nutritional status induces chondrocyte death, which emphasizes the role of chondrocyte apoptosis in cartilage damage and progression of KBD.</p>

Posture evoked response detecting in normal lower limb muscle and its influencing factors / 法医学杂志

OBJECTIVE@#To analyze the latency of posture evoked response of normal lower limb muscle in different stimulations and explore its influencing factors.@*METHODS@#The normal lower limb was induced to produce postural evoked response by the dynamic posturography through two kinds of perturbations, the supporting surface rotation stimulation (Toes-up and Toes-down) and the horizontal perturbation stimulation (Forward and Backward). The latencies of tibialis anterior muscle and gastrocnemius muscle were recorded by surface electromyography acquisition system. The differences of the left and right limb, gender and height on the latency of postural evoked response were analyzed.@*RESULTS@#(1) Under the Toes-up and Backward perturbation, the latency of tibialis anterior muscle was longer than gastrocnemius muscle; under the Toes-down and Forward perturbation, the latency of gastrocnemius muscle was longer than tibialis anterior muscle. (2) The latencies of left limb and right limb had no significant difference. (3) The latency in male was longer than that in female. (4) The latency gradually increased with the increase of height.@*CONCLUSION@#In the postural evoked response, different perturbations, gender and height have significant impacts on the latency of posture evoked response of lower limb muscle. However, the effect of height and gender should be not considered referring to the same individual.

Function disorder assessment on patients with mild psychiatric impairment due to road traffic accidents / 法医学杂志

OBJECTIVE@#To explore the methods to assess the function disorder of patients with mild psychiatric impairment due to road traffic accidents.@*METHODS@#In contrast to that of the patient with physical impairment, the function disorder of the patient with mild psychiatric impairment was assessed by the standard assessment instruments, such as the Barthel index of ADL (BI), Functional Activities Questionnaire (FAQ), Social Disability Screening Schedule (SDSS), Activity of Daily Living Scale (ADL), Physical Self-maintenance Scale (PSMS), and Instrumental Activities of Daily Living Scale (IADL).@*RESULTS@#Except BI, the each total score of FAQ, SDSS, ADL, PSMS, and IADL correlated well with the rank of psychiatric impairment. The difference of each total score of rating scales among different physical impairment rank was not statistically significant. At the impairment rank of level 10, the difference of each total score of FAQ and ADL was not significant between the two groups; each total score of BI, SDSS, and IADL in psychiatric impairment was higher than that of physical impairment, while the total score of PSMS in psychiatric impairment was lower than that of physical impairment. At the level 9, except PSMS, the each total score of BI, FAQ, SDSS, IADL and ADL in psychiatric impairment was higher than that of physical impairment. At the level 8, except BI and PSMS, the each total score of FAQ, SDSS, IADL and ADL in psychiatric impairment was higher than that of physical impairment.@*CONCLUSION@#The different criteria for assessment of mild psychiatric and physical impairment may exist, and appropriate indexes for assessment between physical and psychiatric impairment need to be further elucidated.

Characteristics of computed dynamic posturography of patients with peripheral vertigo / 法医学杂志

OBJECTIVE@#To quantify the posture control ability of patients with peripheral vertigo by computed dynamic posturography (CDP).@*METHODS@#Ninety-one subjects diagnosed with peripheral vertigo by caloric test were divided into unilateral vestibular dysfunction group (unilateral group) and bilateral vestibular disorder dysfunction group (bilateral group). CDP tests including sensory organization test (SOT) and motor control test (MCT) were performed on all subjects. The CDP results were compared with the normal data by t-test.@*RESULTS@#In unilateral group, there was no statistically significant difference in SOT compared with normal data (P > 0.05). In bilateral group, the composite equilibrium score was lower than the normal data, especially during the moving of the platform and the changes of visual stimulation (P < 0.05). In MCT test, the latencies of both groups showed no statistically significant difference compared with normal data (P > 0.05).@*CONCLUSION@#During the non-acute period of peripheral vertigo, patients could maintain static equilibrium. Compared with normal people, dynamic equilibrium function is normal in patients with unilateral vestibular dysfunction, but declined in patients with bilateral vestibular dysfunction.

Forensic medical identification of 355 cases with hearing impairment / 法医学杂志

OBJECTIVE@#To analyze the difference of subjective hearing threshold and objective hearing threshold, and to discuss the importance of standard for hearing evaluation in forensic medicine.@*METHODS@#Three hundred and fifty-five cases (387 ears) of forensic medical identification with hearing impairment were retrospectively analyzed including the items entrusted and hearing test results. All cases were collected from 2004 to 2012 in the forensic science center.@*RESULTS@#In the 387 ears, 218 ears (56.3%) were evaluated the degree of disability and 106 ears (27.4%) were identified the degree of damage. In the disability degree evaluation, the subjective hearing threshold and the objective hearing threshold were significant different in 120 ears (55.0%), while in damage degree evaluation, the subjective hearing threshold and the objective hearing threshold were significant different in 69 ears (65.1%).@*CONCLUSION@#Because of camouflaging or exaggerating the hearing impairment by the wounded, the subjective hearing threshold can't accurately assess the existence and the degree of hearing impairment. In the forensic identification, auditory brainstem response, 40 Hz auditory event related potential and auditory steady-state response should be combined in the application to evaluate the hearing impairment for the wounded in order to ensure the reliability of the evaluation of hearing impairment.

Stature estimation based on the length of tibia and fibula measured by digital X-ray in Chinese Han teenagers / 法医学杂志

OBJECTIVE@#To derive regression formulae for stature estimation using forensic radiography from the tibia and fibula of Chinese Han teenagers in Sichuan Province.@*METHODS@#To construct equations, measurements were conducted on the training sample (412 adults, 201 males and 211 females). The whole length of the fibula and four measurements of tibia were determined using CR radiography, rectified through theoretical magnification. The regression formulae were relatively constructed to the real stature measured in an erect position. Through using the testing sample (40 adults) for the regression formulae, the reliability of the regression formulae was assessed.@*RESULTS@#The range of correlation coefficients of four measurements for tibia was 0.880-0.895 in the sex-unknown group, 0.869-0.893 in the male, and 0.845-0.855 in the female. The five measurements were found to be better correlated with stature in the male than in the female.@*CONCLUSION@#The digital X-ray of the tibia and fibula for stature estimation is proved to be effective in forensic individual identification; therefore, these equations can be of great assistance to the stature estimation of the contemporary Chinese Han teenagers.

Comparisons among three frequency-specific auditory evoked potentials in normal hearing adults / 法医学杂志

OBJECTIVE@#To provide supports for the application of auditory evoked potential (AEP) in the evaluation of behavioral threshold, by studying the difference and relevance between the pure tone audiometry (PTA) and three frequency-specific auditory evoked potentials, including 40 Hz auditory event related potentials (40 Hz AERP), tone burst auditory brainstem response (Tb-ABR) and auditory steady-state response (ASSR).@*METHODS@#Three frequency-specific AEP and PTA thresholds were recorded at speech frequency (0.5, 1, 2, 4 kHz) from thirty-four adults with normal hearing (68 ears). Then, the relationship between the AEP thresholds and PTA thresholds were analyzed respectively.@*RESULTS@#There were good correlations between three frequency-specific AEP thresholds and PTA thresholds at speech frequency. However, the difference of thresholds between each frequency-specific AEP and PTA was not same. The difference of thresholds were the smallest and the relevance were the best between 40 Hz AERP and PTA at 0.5 kHz, and between ASSR and PTA at 2, 4 kHz. At 1 kHz, there were not statistical difference between ASSR, 40 Hz AERP and PTA, while the relevance of 40 Hz AERP was better than ASSR.@*CONCLUSION@#Different methods should be chosen to assess the objective behavioral thresholds at different frequency.

Identifying malingering symptoms of balance disorders by computerized dynamic posturography / 法医学杂志

OBJECTIVE@#To develop an objective criteria with computerized dynamic posturography (CDP) for identification of non-organic or malingering symptoms of balance disorders.@*METHODS@#One hundred and four normal subjects were instructed to feign balance disturbance after naturally performing on CDP. Paired t-test was used to determine if there was significant difference of CDP equilibrium scores between normal and malingering performance. The CDP records including raw curve of 50 subjects were critically evaluated by previously published seven criteria in indicating malingering performance. If the sensitivity of a criterion was greater than 90%, it would be accepted as our laboratory's identifying criterion. The CDP records of remaining 54 subjects were used to test the sensitivity and specificity of the accepted criteria.@*RESULTS@#There were statistically significant differences of CDP equilibrium scores between normal and malingering performance (P < 0.01). The mean equilibrium scores of malingering performance were lower than that of normal performance. The sensitivity and specificity of our criteria for identifying malingerers were 92.5% and 98.2%, respectively.@*CONCLUSION@#It is necessary to identify malingering performance before analyzing the CDP equilibrium scores. Our identifying criteria of malingerers seems to be quite useful and accurate.

Application of slow vertex response in auditory threshold prediction for subjects with hearing loss / 法医学杂志

OBJECTIVE@#To study the value of slow vertex response (SVR) in the evaluation of hearing loss by comparing the hearing thresholds acquired with SVR and pure tone audiometry (PTA).@*METHODS@#Twenty-five subjects (40 ears) with sensorineural hearing loss were tested by PTA and SVR. According to the thresholds of PTA, these ears were subdivided into mild, moderate and severe hearing loss groups, and rank sum test was performed on the thresholds of SVR and PTA for all the hearing loss groups. Then, the correlation between PTA thresholds and SVR thresholds was analyzed and the mathematical models were established for predicting behavioral thresholds by the thresholds of SVR.@*RESULTS@#At four test frequencies (0.5, 1, 2 and 4kHz), the thresholds of SVR had high correlations with thresholds of PTA. Four liner regression equations were established, and the correlation coefficient(r) were 0.971, 0.976, 0.957 and 0.928, respectively (P < 0.05). Back substitution test showed that the liner regression equations would be an easy method for estimating the behavior thresholds.@*CONCLUSION@#The behavioral threshold can be well judged and evaluated by the liner regression equations established with SVR thresholds.

Assessment of dynamic posture equilibrium function after traumatic brain injury / 法医学杂志

OBJECTIVE@#To explore characteristics and causes of equilibrium function deficits after traumatic brain injury(TBI).@*METHODS@#Ninety-five patients after TBI in traffic accidents were tested using computerized dynamic posturography (CDP). The CDP findings of patients were compared with normal value. The patients were grouped based on TBI and audition disorders degrees. The results were compared within the patients groups.@*RESULTS@#The equilibrium scores of the TBI group were significantly lower than the normal value. The utilization rate decreased for vision and vestibular sensation information in the TBI group, especially for vestibular sensation. The primary TBI degree and audition decrease had no significant affect on posture stability.@*CONCLUSION@#The poor posture stability was observed in TBI patients without limbs disturbance. The response and adaptation abilities decrease in these patients. This could be explained by the damage to peripheral vestibular and central balance structures.

The progress in the study on slow vertex response / 法医学杂志

Slow vertex response (SVR) is one of long latency auditory evoked potentials. It is a biological and electric response originating from brain cortical neuron evoked by sound stimulus with the latency from 50 to 500 milliseconds. Of all the neuroelectric physiological audiometries, it is the earliest method applied in assessing the function of the auditory neural conduction pathway. The concept, neural generators of SVR have been introduced in this article. Influencing factors on SVR were discussed such as stimulus parameters, consciousness state, age, maturation of the subject. Applications of SVR in clinical and forensic medicine identification were also discussed.

Comparison of thresholds acquired with SVR and PTA in normal hearing subjects / 法医学杂志

OBJECTIVE@#To investigate the value of slow vertex response (SVR) for forensic appraisement of hearing dysfunction by comparing the thresholds acquired with SVR and pure tone audiometry (PTA).@*METHODS@#Forty-six subjects with normal hearing were tested with PTA (0.125-8 kHz) and SVR (0.5-4 kHz). Paired t-test analysis was performed on the thresholds of SVR and PTA at each frequency (0.5, 1, 2 and 4 kHz), and analysis of variance was performed on the difference between the pure tone audiometry threshold and the evoked potential threshold among different frequency.@*RESULTS@#At each frequency(0.5, 1, 2 kHz and 4 kHz), difference between the thresholds acquired with SVR and PTA was statistically significant(P < 0.05). The mean difference of the two thresholds were (5.98 +/- 6.72) dB HL at 0.5 kHz, (3.86 +/- 6.12) dB HL at 1 kHz, (7.12 +/- 6.56) dB HL at 2 kHz, (8.53 +/- 7.90) dB HL at 4kHz, and the mean difference at 1 kHz was the smallest. This variation between frequencies was also statistically significant.@*CONCLUSION@#The thresholds acquired with SVR were higher than those acquired with PTA. The adjusted thresholds should be established at each frequency, when the SVR thresholds are used to evaluate the behavioural hearing thresholds.

Psychological nursing after cleft lip and palate surgery with precardium area pain: a case report / 华西口腔医学杂志

A 18-year-old female patient after cleft lip and palate surgery with an uncommon complication-precardium area pain were reported. The psychological treatment and nursing along with routine clinical treatment to the patient were applied. The patient had recovered from the precardium area pain after one week treatment.